User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/09/17

Biomaterials Design Lab

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Objective Prep AuNP samples for AA analysis tomorrow. Description I made two stock solutions Au: 20.3mg of HAuCl4·H2O in 24mL of water (2.36mM) and BSA: 47.2mg of BSA in 45mL of water (15.7uM). Samples were made in the following manner using similar ratios to the experiments I ran on May 23rd I made 2 solutions (11.8 mg of HAuCl4 in 14 mL of water and 23.2 mg of Bovine Serum Albumin in 22 mL of water) and used these solutions to make samples for preparing AuNPs. } Data Notes Approx. Au/BSA ratio Volume Au added (mL) Volume BSA added (mL) Volume Water added (mL) Actual Ratio 20 1.00 8.334 0.666 18.0 30 1.00 5.554 3.446 27.1 40 1.00 4.166 4.834 36.1 50 1.00 3.334 5.666 45.1 60 1.000 2.778 6.222 54.1 70 1.00 2.38 6.62 63.1 80 1.00 2.084 6.916 72.1 90 1.00 1.854 7.146 81.1 100 1.00 1.666 7.334 90.2 110 1.00 1.514 7.486 99.3 120 1.00 1.386 7.614 108.5 130 1.00 1.28 7.72 117.4 140 1.00 1.188 7.812 126.5 150 1.00 1.112 7.888 135.2 160 1.000 1.04 7.96 144.5 170 1.00 0.98 8.02 153.4