User:Mark X. Ling/Notebook/Micb 323/2009/02/10

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Experiment 5: Part1: Gel Electrophoresis of Modified Restriction Enzyme Part 2: Southern Transfer Part 3: Protein Assay(Bradford assay and BCA assay) Bradford Assay Standard. Tube # BSA (uL) H2O (uL) 1 0 100 2 10 90 3 15 85 4 20 80 5 25 75 6 30 70 7 35 65 8 40 60 Microwell Binichoninic ACid (BCA) Assay Standard. BCA conc (ug/mL) BCA stock (uL) Water (uL) 2000 250 0 1502 188 60 1000 125 125 752 94 160 500 62.5 187.5 252 31.5 218.5 124 15.5 234.5 24 3 247 Post Lab comments Big mistake, added SDS to lysate did not do Bradford of recombinant only did BCA but result was not good