User:Mara Peterson/Notebook/Mara Peterson/2015/12/16
|Fall 2015|| Main project page|
Performing plasmid minipreps on the liquid cultures started yesterday, quantifying the DNA yield, performing restriction digests to verify presence of insert, and sending promising samples out for sequencing.
Followed the Sigma kit instructions. Eluted in 50 µL nuclease-free H2O.
Using 1 µL (~500 ng) of each plasmid as the template.
Incubate at 37 °C for 5 minutes, then run on the gel, 100 V for 50 minutes.
See first six sample lanes. Both HA1 samples appear to have the insert (~100 bp), and EM7:ZeoR samples 1 and 2 appear to have the insert (~500 bp). Will send those four samples to SOLS Core lab for sequencing using P0001 primer.