User:Mar/Notebook/2007-6-25

mouse cortical extract (cont)

I added 1.5mL of RIPA to the pellet, sonicated, combined, mixed and assayed:

1.371mg/mL in 3.0 mL => 16.5µg/punch

Western blot

Run using 3-8% Tris-Acetate cassette at 1.8µL/lane (=2.5µg). Run @ standard parameters for 35+15+10min Then transferred onto nitrocellulose for 3 hrs (deviation: at first transfer buffer without MeOH and antioxidant was used, it was noticed and buffer was replaced with the right one just after start).