User:Madeline Hartman/Notebook/Dr. Hartings Lab/2014/02/20

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Entry title Ran a trial in OceanView for Absorbance readings. Run at 70°C set through Qpod Run A Wizard Select spectroscopy JAZ is the correct hardware device. Click next Select absorbance and click next Select integration time as 1 ms Select scans to average 10 Click next Open lamp. Click lightbulb to store reference spectrum. Click next Close lamp. Click the lightbulb to store dark spectrum. Click finish Open lamp. Click save graph to files. Configure Save the first available scan every 1 minute Stop after 2 hours Save to directory in a dropbox folder - 02-20-H2O-70 File format- Tab separated with headers Create file name - mb_opd_acetate_h202_h20_70_ Click apply When ready to run, click play button, then click the save graph to files button a second time. Cuvette at start time contains- 0.675 mL (2.8 mg OPD in 2.8 mL H2O) 0.75 mL dilute H2O2 (0.1135 μL H2O2 and 0.8865 μL H2O) First reading taken after 45 seconds. 0.225 mL of Mb added after 2 minutes (between readings 2 and 3) (1 mg Mb/KCl/Acetate buffer in 1 mL H2O) Steps were repeated at same temperature with 1 mg Mb/KCl/Citrate buffer in 1 mL H2O