User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/02/18

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PVA-Alg Bead Synthesis Main project page
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February 18, 2015

  • Run normal-scale PVA-Alg-NaMT with no citric acid
  • Mass out upscaled product, solvent washing and FTIR, pXRD

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)

PVA-Alg-NaMT (no citric acid)

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of NaMT and ~0.05 g AC should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of saturated citric acid with 4% calcium chloride
    1. Add 8.4 g (4%) calcium chloride to 210 mL of water
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
  5. Stir for 2 hrs to allow solidification
  6. Rinse with water to remove excess reactants

Measurements

  • PVA-Alg-NaMT (no citric acid)
    • PVA: 0.99 g
    • Alg: 0.16 g
    • NaMT: 0.1 g
    • CaCl2: 8.5 g
  • Mass of product: ~2.93 g

Notes

  • Try one (maybe with halocite beads):
    • Surfactant (and also buffer) > pH??
    • Add sugar (sorbitol or manitol)
    • Start with ammonium based (choline chloride)


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