User:Madeleine Y. Bee/Notebook/CHEM-581 Experimental Chemistry/2014/10/03

CHEM 581: Experimental Chemistry

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October 3, 2014 Procedures New Film Synthesis PVA film 1.0011 g PVA PVA Clay film g PVA g NaMT NaMT Cation Exchange Assuming 92 miliequivalents (meq) of Na+ per 100g of montmorillonite.<br.> Tributylhexadecylphhosphonium bromide exchange Add 1.12g of Sodium Montmorillonite (NaMT) to a scintillation vial containing 20mL of 50:50 HPLC grade water:ethanol. (1.12 g NaMT)×(92×10-3 eq/100 g NaMT)×(507.65 g/mol tributylhexadecylphhosphonium bromide)=0.523 g tributylhexadecylphosphonium bromide Add 0.523g of tributylhexadecylphosphonium bromide to exchange 100% of the Na from the clay Cap the vial Stir for 1 week Vacuum filter using 0.2uM nylon filter paper Dry in oven at 90C overnight Grind with a mortar and pestle Store in a dessicator Choline chloride exchange Add 1.12g of Sodium Montmorillonite (NaMT) to a scintillation vial containing 20mL of 50:50 HPLC grade water:ethanol. (1.12 g NaMT)×(92×10-3 eq/100 g NaMT)×(139.62 g/mol choline chloride)=0.144 g choline chloride Add 0.144 g of choline chloride to exchange 100% of the Na from the clay Cap the vial Stir for 1 week Vacuum filter using 0.2uM nylon filter paper Dry in oven at 90C overnight Grind with a mortar and pestle Store in a dessicator Dilutions and Fluoresence Measurements of R6G Solutions PVA film Original concentration: 20uM > 0.1uM (50uL in 10mL) Original: 10uM > 0.1uM (50uL in 10mL) Original: 1uM > 0.5uM (5mL in 10mL) PVA Clay film Original concentration: 20uM > 0.1uM (50uL in 10mL) Original: 10uM > 0.25uM (250uL in 10mL) Original: 1uM > 0.5uM (5mL in 10mL) Data Diluted solutions absorbance:<br.> <br.> Calculating solutions actual concentration from R6G calibration curve constructed on 10/01/2014:<br.>