User:Madeleine Y. Bee/Notebook/CHEM-581 Experimental Chemistry/2014/10/03

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October 3, 2014

Procedures

New Film Synthesis

  • PVA film
    • 1.0011 g PVA
  • PVA Clay film
    • g PVA
    • g NaMT

NaMT Cation Exchange

Assuming 92 miliequivalents (meq) of Na+ per 100g of montmorillonite.

  • Tributylhexadecylphhosphonium bromide exchange
    1. Add 1.12g of Sodium Montmorillonite (NaMT) to a scintillation vial containing 20mL of 50:50 HPLC grade water:ethanol.
      • (1.12 g NaMT)×(92×10-3 eq/100 g NaMT)×(507.65 g/mol tributylhexadecylphhosphonium bromide)=0.523 g tributylhexadecylphosphonium bromide
    2. Add 0.523g of tributylhexadecylphosphonium bromide to exchange 100% of the Na from the clay
    3. Cap the vial
    4. Stir for 1 week
    5. Vacuum filter using 0.2uM nylon filter paper
    6. Dry in oven at 90C overnight
    7. Grind with a mortar and pestle
    8. Store in a dessicator
  • Choline chloride exchange
    1. Add 1.12g of Sodium Montmorillonite (NaMT) to a scintillation vial containing 20mL of 50:50 HPLC grade water:ethanol.
      • (1.12 g NaMT)×(92×10-3 eq/100 g NaMT)×(139.62 g/mol choline chloride)=0.144 g choline chloride
    2. Add 0.144 g of choline chloride to exchange 100% of the Na from the clay
    3. Cap the vial
    4. Stir for 1 week
    5. Vacuum filter using 0.2uM nylon filter paper
    6. Dry in oven at 90C overnight
    7. Grind with a mortar and pestle
    8. Store in a dessicator

Dilutions and Fluoresence Measurements of R6G Solutions

  • PVA film
    • Original concentration: 20uM > 0.1uM (50uL in 10mL)
    • Original: 10uM > 0.1uM (50uL in 10mL)
    • Original: 1uM > 0.5uM (5mL in 10mL)
  • PVA Clay film
    • Original concentration: 20uM > 0.1uM (50uL in 10mL)
    • Original: 10uM > 0.25uM (250uL in 10mL)
    • Original: 1uM > 0.5uM (5mL in 10mL)

Data

Diluted solutions absorbance:
Image:2014_1003_R6G_solutions_abs.PNG
Calculating solutions actual concentration from R6G calibration curve constructed on 10/01/2014:



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