The camera can dump all the photos it has taken with the push of one button
The destination folder on Larry's computer is in the C: drive
It also sub categorizes the photos by date taken
As for a long usb cable, Im pretty sure they make em almost as long as you would ever want
The camera uses usb mini-b, so we may want to get an adapter (the thought being get a really long usb A cord and get an A to mini b adaptor, that way the long cord can be used for other things as well)
Amazon has usb A extension cables (15-20ft) for under $10
So Koch ordered two Canon Cameras and wants me to try to get the second one setup
Round 2
I am uninstalling the software from last time b/c I had trouble installing it the first go
Actually, having a hard time uninstalling the software...stupid Canon
Ok, all uninstalled...lets see if it will let me reinstall all the software
Had a problem yesterday of installing and then the program crashed. It must have left some residue behind since it says that everything is all nice and installed, yet it was not there
The "Photostitch" program is buggy. It tells me that it has already been installed, then crashes the installer when it fails. I can get around this by intalling manually and then telling it not to mess with photostitch
It seems that I have the "essential" software installed
Looks like it does not come with a memory card, hope thats ok
The new battery is uncharged, using one from old cam
Setting up wireless
SSID Kochlab
security WPA-PSK-TKIP
key Andy Maloney 00:17, 8 July 2009 (EDT): Really? You put the key on the public wiki? I know we are an open lab but don't you think that's a bit much?
Linh N Le 01:07, 8 July 2009 (EDT)Oh shoot! I'm sorry, i totally spaced that I did that. I just wanted to document what I wrote down so that if the camera didn't connect it could have been traced to a bad password. I gotta keep reminding myself that this is a public wiki
Got that setup but when trying to connect this battery also died
May need to wait for a charge tomorrow
Kinesin Activity Assay
Brigette wanted to try to do the phosphorous assay kit that we got from cytoskeleton
From what I know about this, we mix up tubes and ATP and let it sit at different times and measure the phosphorus levels
We then mix our kinesin and the tubes and ATP, let sit at diff times, and re measure the phosphorus
Right now, we are going to polymerize some non-labeled tubes
Brigette is writing up some procedures which I hope to copy from her and paste in my notebook
Procedure
Taking note of what we did as we do it:
1 aliquot of the unlabeled tubes
They are in the -80c in the large falcon tubes
Polymerize them for 20min @ 37C
Add 99ul of BRB80T for a total of 100 ul of tubulin
note: the BRB80T is cold in the 4c fridge, so i warmed the tube by hand for ~5mins
Tubes + brb80t created at 12:10pm
Brigette is making a little bit of everything to check and see if any of it ends up killing the kinesin
We are doing just tubes, just kinesin, tubs + kinesin, the motillity assay with casein, etc
The idea here is to make different kinds of samples and let them all sit for about the same amount of time (~30mins)
Samples to be made:
30ul MT + 30ul Kin
30ul MT + 30ul Kin + .6ul ATP (1uM ATP)
30ul MT + 30ul BRB80T + .6ul ATP (1uM ATP)
30ul MT + 30ul Kin + 1.2ul ATP (2uM ATP)
30ul MT + 30ul BRB80T + 1.2ul ATP (2uM ATP)
Note: When i say MT I mean MT+AF (motillity soln w/o casein)
Second Note: some drops got stuck to the side walls of the tubes, so Brigette washed them down with a couple ul of BRB80
We let the samples sit for ~10mins to allow the reactions to complete (since adding the dye and quencher stop the reactions all together)
Turns out it is over diluted and Brigette didn't get any useful readings from it