User:Linh N Le/Notebook/2009/06/01/Microtubules

Microtubules

• We made MTs today
• First we took an aliquot (2ul) of FITC tubulin and warmed it in the PCR
  • This caused the tubulin to polymerize into microtubules
  • We also added a buffer, 198 ul of room temperature BRB80T, to stabilize them
• We then imaged them in the microscope
  • We saw 100's of tubes (although they are smaller than what Koch made last week http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/05/26/FITC_Microtubules_with_antifade)
• We then made a batch with Antifade
  • The old antifade from last week was no good, but a fresh batch came out great!