User:Linh N Le/Notebook/2009/06/01/Microtubules

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Microtubules

  • We made MTs today
  • First we took an aliquot (2ul) of FITC tubulin and warmed it in the PCR
    • This caused the tubulin to polymerize into microtubules
    • We also added a buffer, 198 ul of room temperature BRB80T, to stabilize them
  • We then imaged them in the microscope
  • We then made a batch with Antifade
    • The old antifade from last week was no good, but a fresh batch came out great!
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