LEAH TOLOSA, Ph.D.
Center for Advanced Sensor Technology
University of Maryland Baltimore County
1000 Hilltop Circle, Baltimore, MD 21050
410-455-3432 (office), 410-455-6500 (fax)
[Printable Version: Tolosa CV pdf]
University of Connecticut, Storrs 
University of Santo Tomas, Manila 
University of Santo Tomas, Manila 
2006 – present University of Maryland Baltimore County
2004 - present UMBC Center for Advanced Sensor Technology
2001 – 2006 University of Maryland Baltimore County
1996 – 2001 University of Maryland Baltimore
1994 – 1996 Brandeis University, Waltham, MA
1988 – 1994 University of Connecticut, Storrs, CT
1980 – 1987 University of Santo Tomas, Manila
- American Chemical Society
- American Association for the Advancement of Science
- Philippine-American Academy of Science and Engineering, Member, Board of Directors
- Biomedical Engineering Society
- Network of Minority Research Investigators, Member, Oversight Committee
2005-2010: Principal Investigator, Research Project Grant (R01DK072465-01) from the National Institutes of Health - $1,359,750
2004-2007: Principal Investigator, Technologies for Metabolic Monitoring (W81XWH-04-1-0781) from the US Army Medical Research and Materiel Command - $299,850
2003 – 2006: Principal Investigator, Mentored Quantitative Research Career Development Award (K25DK062990) from the National Institutes of Health - $388,000
- Lakowicz, J. R., Tolosa, L., Eichhorn, L., Rao, G. (March 6, 2001) Engineered Proteins for Analyte Sensing, Patent No. US 6, 179,534 B1.
- Tolosa, L., Ge, X., Rao, G. (patent pending) Proteins, Sensors, and Methods of Characterizing Analytes Using the Same.
- L. Tolosa, G. Rao, I. Kostov, X. Ge (patent pending) Multianalyte Optical Sensing System Using Novel Proteins as Biosensing Elements.
- L. Tolosa, G. Rao, Y. Kostov, R. Badugu (disclosure) Minimally-invasive monitoring of the effectiveness of cancer chemotherapy.
- L. Tolosa, G. Rao, R. Badugu (disclosure) Modulation and detection of protein conformation with probes containing a photochemical switch.
- L. Tolosa, A. Bartolome, H. Lam (disclosure) Dicysteine binding protein mutants for site-selective fluorescence labeling and immobilization on chemically modified glass.
- L. Tolosa, H. Lam, Y. Kostov, G. Rao (patent application) Fluorophore Based Temperature Sensor
Selected Journal Publications
- Bartolome, A., Smalls-Mantey, L., Lin, D., Rao, G., Tolosa, L. “FRET-based glucose monitoring for bioprocessing”, Genetically Engineered Probes for Biomedical Applications, Proc. of SPIE, 6098, 60980L-1 – 8, 2006.
- Kostov, Y., Smith, D. S., Tolosa, L., Rao, G., Gryczynski, I., Gryczynski, Z., Malicka, J., Lakowicz, J. R. (2005) Directional Surface Plasmon-Coupled Emission from a 3 nm Green Fluorescent Protein Monolayer, Biotech. Prog., 21, 1731-1735.
- Bartolome, A., Bardliving, C., Rao, G., Tolosa, L. (2005) Fatty acid sensor for low-cost lifetime-assisted ratiometric sensing using a fluorescent fatty acid binding protein, Anal. Biochem., 345, 133-139.
- Ge, X., Tolosa, L. (corresponding author), Rao, G. (2004) Dual-labeled glucose binding protein for ratiometric measurements of glucose, Anal. Chem., 76, 1403-1410.
- Ge, X., Tolosa, L., Simpson, J., Rao, G. (2003) Genetically engineered binding proteins as biosensors for fermentation and cell culture, Biotech. Bioeng., 84, 723-731.
- Tolosa, L., Ge, X., Kostov, Y., Lakowicz, J. R., Rao, G. (2003) Dual-emitting biosensors for glucose and glutamine from genetically engineered E. coli binding proteins, Proc. SPIE Genetically Engineered and Optical Probes for Biomedical Applications, 4967, 19-25.
- Kostov, Y., Tolosa, L., O’Connell, K., Anderson, P., Liu, Y., van Beek, N., Rao, G. (2003), Monitoring DsRed protein concentration in frozen insect larvae, Proc. SPIE Genetically Engineered and Optical Probes for Biomedical Applications, 4967, 100-107.
- Tolosa, L., Ge, X., Rao, G., (2003) Reagentless optical sensing of glutamine using a dual-emitting glutamine-binding protein, Anal. Biochem., 314, 199-205.
- Tolosa, L., Kostov, Y., Harms, P., Rao, G. (2002) Non-invasive measurement of dissolved oxygen in shake flasks, Biotech. Bioeng., 80, 594-597.
- Murtaza, Z., Tolosa, L., Harms, P. and Lakowicz, J. R. (2002) On the Possibility of Glucose Sensing Using Boronic Acid and Luminescent Ruthenium Metal-Ligand Complex, J. Fluorescence, 12(2), 187-192.
- Lakowicz, J.R., Gryczynski, I., Piszczek, G., Tolosa, L., Nair, R., Johnson, M.L., Nowaczyk, K. (2000) Microsecond dynamics of biological macromolecules, Methods in Enzymology, 23, 473-509.
- L. Tolosa, K. Nowaczyk, J. R. Lakowicz, Fluorescence Probes for Biochemical Systems in An Introduction to Laser Spectroscopy, 2nd edition (2002), pp. 139 – 171, D. L. Andrews and A. A. Demidov, eds., Plenum Publishing Corp.
- L. Tolosa, Y. Kostov, G. Rao, Fluorescence Based Sensing for Bioprocess Monitoring, in Fluorescence Sensors and Biosensors (2005), R. Thompson, ed., Marcel Dekker Publishers.
- L. Tolosa, G. Rao, Binding Proteins as Glucose Sensors, in Glucose Sensing, Topics in Fluorescence Spectroscopy, Vol. 11, (2006) C. Geddes and J. R. Lakowicz, eds., Springer-Verlag.
- L. Tolosa (Invited Talk) Biosensing at the UMBC Center for Advanced Sensor Technology, Spectrx, Inc., November 2, 2006, Norcross, GA.
- L. Tolosa (Invited Talk) From the Winged Monster to Diabetes, University of Connecticut, Celebrating the Elements of Connecticut Chemistry, September 29, 2006, Storrs, CT.
- L. Tolosa (Oral Presentation) A low-cost reagentless multianalyte metabolic monitor, The 9th World Congress on Biosensors, May 10 – 12, 2006, Toronto, Canada.
- L. Tolosa (Invited Talk) A Low-Cost Multi-Metabolite Monitor for Diabetes Care, Biomedical Engineering & Institute for Micromanufacturing, Louisiana Tech. University, May 1, 2006, Ruston, LA.
- L. Tolosa (Poster) Designer Protein Biosensors for Low-Cost, Point-of-Care Diagnostics, Network of Minority Research Investigators Workshop, April 20 – 21, 2006, Bethesda, MD.
- A. Bartolome, D. Lin, G. Rao and L. Tolosa (Poster) Fluorescence detection of glucose based on glucose binding protein immobilized on dextran modified glass capillary, Pittsburgh Conference, March 12-17, 2006, Orlando, FL.
- A. Bartolome, Y. Kostov, G. Rao and L. Tolosa (Poster) Designer Protein Biosensors for Low-Cost, Point-of-Care Diagnostics, Gordon Research Conference on Bioanalytical Sensors, February 26 – March 3, 2006, Ventura, CA.
- A. Bartolome, L. Smalls-Mantey, D. Lin, G.Rao and L.Tolosa (Poster) FRET-based glucose monitoring for bioprocessing, SPIE Photonics West, January 21 – 26, 2006, San Jose, CA
- L. Tolosa, A. Bartolome, C. Bardliving (Poster) Improved Ratiometric Sensing of Fatty Acids, 5th Diabetes Technology Meeting, November 10-12, 2005, San Francisco, CA.
- L. Tolosa (Poster) Binding Proteins for Low-Cost Metabolite Sensing, The Gordon Research Conference on Chemical Sensors and Interfacial Design, August 28 – September 2, 2005, Oxford, UK.
- L. Tolosa (Invited Talk) Practical Fluorescence, University of San Carlos – PAASE Tutorials, June 27, 2005, Cebu, Philippines.
- L. Tolosa (Invited Talk), A Low-Cost Reagentless Multi-Analyte Metabolic Monitor, Philippine-American Academy of Science and Engineering Annual Meeting, June 24 – 25, 2005, Cebu, Philippines.
- L. Tolosa (Invited Talk), Biosensor Design for Point-of-Care Diagnostics, University of Santo Tomas - PAASE Symposium, June 22, 2005, Manila, Philippines.
- L. Tolosa (Poster), A. Bartolome, Y. Kostov, G. Rao, A Low-Cost Reagentless Multi-Analyte Metabolic Monitor, Pittsburgh Conference, February 26 – March 4, 2005, Orlando, FL.
Current and Previous Support
- Grant # DK062990 (2003-2006): This is a 3-year plus one-year no-cost extension NIH K25 grant under the mentorship of Dr. Govind Rao. This project seeks to develop a glucose sensor based on the E. coli glucose ABC (ATP-binding cassette) transporter. The objective of this study is to explore the potential of groups of proteins as sensors rather than the traditional single proteins. [$388,000]
- Award # W81XWH-04-1-0781 (2004-2006): This is a project funded by the US Army on a low-cost portable system for multi-analyte metabolic monitoring. This system is based on optical sensing of glucose, glutamine and lactate using a disposable cassette with wells containing lyophilized fluorescent binding proteins and a buffer reservoir. The main goal is to assess stress levels of military personnel in the field, but the device is applicable to other conditions such as diabetes monitoring. [$299,850]
– RO1 DK072465 (2005-2010): This project is for an optical device for minimally invasive, continuous monitoring of glucose, glutamine and lactate. Improvements over the disposable sensor are incorporated such as immobilization of the binding proteins, focusing strategies, and redundant measurements. [$1,359,750]
– R01 DK072465-01S1 (2006 – 2007): Research Supplement to Promote Diversity in Health-related Research for Ms. Smalls-Mantey. The grant is support of Ms. Smalls-Mantey for two summers. [$15,915]
– Unrestricted Gift from Becton-Dickinson Technologies for the support of one post-doctoral fellow. [$30,000]
- Participant in Scientific Review Panels for the National Institute of Diabetes and Digestive and Kidney Diseases: Develop new therapies for Type 1 diabetes and its complications (ZDK1 GRB-2, July 30, 2004) and Small Business Bioengineering and Physiology (ZRG1 SSMI-K, March 13 – 14, 2006).
- Participant in the Scientific Review Panel for the US Army Research and Materiel Command: Technologies in Metabolic Monitoring (April 10 – 12, 2005).
- Initiated collaborations between the UMBC Center for Advanced Sensor Technology and the UM Greenebaum Cancer Center, UMBC Center for Advanced Studies in Photonics Research, US Department of Agriculture, Spectrx, Inc., and the Sensor Group of the University of Santo Tomas, Manila.
- An active member of the Philippine-American Academy of Science and Engineering, served as Secretary from 2000 – 2005 and as Member of the Board of Directors from 2006 – 2008. I was a major participant in organizing the PAASE annual meetings from 2001 to 2005 both here in the US and in the Philippines.
- An active reviewer of manuscripts submitted to leading scientific journals and grant proposals submitted to agencies such as the Maryland Industrial Partnerships.
Thesis and Postdoctoral Advisor
- Thesis Advisor: Dr. Challa Vijaya Kumar, Department of Chemistry, University of Connecticut, Storrs, CT
- Postdoctoral Advisor: Dr. Saul G. Cohen (retired), Department of Chemistry, Brandeis University, Waltham, MA
- Dr. Amelita Bartolome (October 1, 2004 – July 30, 2006)
- Dr. Hung Lam (February 10, 2006 to present)