User:Kristina M Holton/Ovarian Notebook 2007 9 14

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Qiagen RNeasy FFPE Kit: G02, G03, Drapkin

10-10 micron samples, split into 2 tubes (50 microns each)

  • Step 5: Well-pelleted, no visible tumor sample in G03
  • Step 7: White & cloudy
  • Step 9: Better pelleted
  • Step 11: Cloudy, using larger volumes
  • Step 12: After 80C, clarified
  • Step 15: 1750 EtOH for G02 as per Supplemental Protocol (too much for tube), 1400 EtOH for G03, Drapkin (fits into tube, original protocol calls for 1300ul), Small amount of precipitate
  • Step 16: G02 requires 4 passes through column
  • Step 21: Eluted in 30ul
QF 9/14/07
ng/ul
260/280
260/230
Vol
Total
Dilution
Total to Illumina
G02
674.89
1.95
1.69
60ul
40ug
30ul + 172ul H20
202ul
G03
879.55
2.02
2.06
60ul
52ug
30ul + 234ul H20
264ul
Drapkin
184.01
1.95
1.76
60ul
11ug
30ul + 25ul H20
55ul
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