User:Kristina M Holton/Ovarian Notebook 2007 9 13

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Ambion Recoverall Kit: G02, G03, Drapkin

10-10 micron samples, split into 2 tubes (50 microns each)

  • Deparaffinization Step 2: still appreciable parafin
    • G03: No visible tumor sample after xylene spin-down
  • Deparafiinization Step 4-1: Add EtOH, vortex = cloudy, poorly pelleted after 2 minute spin-down
  • Deparaffinzation Step 4-2: Spin down 2.5 mins, slightly better

T=3hrs: samples did not fully clarify

Nucleic Acid Isolation Step 2: Only partial clarification after add Isolation Buffer, large amount tissue not digested


AR 9/13/07
ng/ul
260/280
260/230
Vol
Total
Dilution
Total to Illumina
G02
438.36
2.02
1.37
120ul
52ug
60ul + 202ul H2O
262ul
G03
99.45
2.06
.36
120ul
12ug
60ul
60ul
Drapkin
153.59
1.94
.77
120ul
18ug
60ul + 32ul H20
92ul
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