User:Kristina M Holton/Ovarian Notebook/2006-7-17

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BioAnalyzer: NanoChip on FFPE Samples

BioAnalyzer: MicroChip on FFPE Samples


Total & miRNA Extraction Protocol: SW480 & SW620 cell lines

1 plate each

  • Run 1: Used RW1 instead of RWT (from RNeasy Mini Kit)
  • Run 2: Used RWT (miRNeasy kit)
  • Each plate run in 3 columns
Sample
ng/ul
260/280
260/230
Vol
Total
SW620 Run 1
2606
2.1
2.2
180ul
470ug
SW620 Run 2
2576
2.09
2.11
180ul
462ug
SW480 Run 1
1789
2.12
2.20
180ul
322ug
SW480 Run 2
2222
2.09
2.16
180ul
400ug
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