User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/12

AU CHEM-570 Lab Prep

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Bench work Ran DNA gel (1% agarose) with ligation samples from yesterday Lane 1: Ladder (10μL) Lanes 2 & 3 skip Lane 4: 5μL pKK223-3 BamHI digest, ligation (with 10μL in ligation reaction) Lanes 5 & 6 skip Lane 7: 5μL pKK223-3 BamHI digest, ligation (with 15μL in ligation reaction) Lanes 8 & 9 skip Lane 10: 5μL pTXB1 test PCR from 8/10 (with Pfu Turbo buffer 'A') Lanes 11 & 12 skip Lane 13: 5μL pTXB1 test PCR from 8/10 (with Pfu Turbo buffer 'B') Results Gel