User:Karmella Haynes/Notebook/miR Trigger/2011/02/28

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02/28/11

  • ✓ JDS33-47 miR Sensor transfections/ dox induction: check expression



miR Sensor expression in JDS33-47 transient transfections

Construct Expected expr. -dox Expected expr. +dox
1. KAH179/176/MV8: Target +UAS/ Repressor --- YFP-, RFP+, CFP- YFP+, RFP+, CFP-
2. KAH180/176/MV8: Target ---/ Repressor --- YFP-, RFP+, CFP+ YFP+, RFP+, CFP+,
3. KAH179/177/MV8: Target +UAS/ Repressor m-target YFP-, RFP+, CFP- YFP+, RFP-, CFP+
4. KAH180/177/MV8: Target ---/ Repressor m-target YFP-, RFP+, CFP+ YFP+, RFP-, CFP+
5. KAH179/178/MV8: Target +UAS/ Repressor s-target YFP-, RFP+, CFP- YFP+, RFP-, CFP+
6. KAH180/178/MV8: Target ---/ Repressor s-target YFP-, RFP+, CFP+ YFP+, RFP-, CFP+

Microscopy 2/28/11



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