User:Karmella Haynes/Notebook/miR Trigger/2010/08/17

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08/17/10

  • ✓ New idea: three-color miR sensor to report different levels of miR expression
  • ✓ Order plasmid: phGluc (22522) from Addgene (to make new miR-luc gene, w/o YFP)



New idea: three-color miR sensor to report different levels of miR expression
> Scheme: RFP-Repressor-miRtarget silences YFP-10xGal4-Reporter #1 and CFP-15xGal4-Reporter #2
--> Will need to experiment with varying Gal4 copy number to get different sensitivities

> Need to modify miR-luc gene so that it does not need YFP
> Use secreted reporter Gaussia luciferase, mammalian expression optimized (see http://www.addgene.org/pgvec1?f=c&identifier=22522&atqx=Gluc&cmd=findpl)
--> Has no BB sites!



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