User:Karmella Haynes/Notebook/miR Trigger/2010/02/16

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02/16/10

  • ✓ RFP knock-down experiment: plate cells for transfection (tomorrow)

RFP knock-down Expt.
> Plate cells in 6-well dishes (~5.0x105/well):

  1. JDS33-47 (HPK-RFPrep-siRNAtarget, 0 Dox)
  2. JDS33-47 (HPK-RFPrep-siRNAtarget, 24 hr. Dox)
  3. JDS33-47 (HPK-RFPrep-siRNAtarget, 48 hr. Dox)
  4. JDS33-47 (HPK-RFPrep ctrl, 0 Dox)
  5. JDS33-47 (HPK-RFPrep ctrl, 24 hr. Dox)
  6. JDS33-47 (HPK-RFPrep ctrl, 48 hr. Dox)

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  1. JDS33-47 (untransfected, 0 Dox)
  2. JDS33-47 (untransfected, 24 hr. Dox)
  3. JDS33-47 (untransfected, 48 hr. Dox)
  4. U2OS plain* (HPK-RFPrep-siRNAtarget)
  5. U2OS plain* (HPK-RFPrep ctrl)
  6. U2OS plain* (untransfected)


  • Use U2OS Flp-in T-REx instead since U2OS cells aren't ready yet (also split 1x 1:10 flask)



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