03/11/11
- ✓ Luc activity assay: 12-day luc rep, 2-day Pc-TF
Luc Activity Assay
Luc activity assay
> Cells: dox-induced Gal4-EED expression/ 2 days, followed by transfection for 4 days
1-6. KAH160/MV1 (human Pc-TF), 0, 50, 100, 200, 400, 800 ng plasmid
7-12. KAH170/MV1 (human Pc-TF), 0, 50, 100, 200, 400, 800 ng plasmid
> Sample processing:
- Add 500 μL medium to cells in rep 1 set (measured before); Resuspend cells in 1 mL medium total
- Aliquot 3x 100 μL samples into 96-well plate
- Save the rest for cell count (Millipore Scepter)
- Normalize each value to cell count
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