User:Karmella Haynes/Notebook/Polycomb project/2010/12/28

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12/28/10

  • ✓ Cell growth assay: seed cultures for growth assay for 126-3, 154-2, 132-8, FTRx
  • ✓ Culture: split all KAH U2OS lines 1:10
  • ✓ ChIP: 128-8, 129-4 DNA purification day 2 (etOH ppt overnight)



Cell growth assay
> To complement B-gal assay
> KAH126-1 done already (images collected) Remaining lines:

  1. KAH126-3
  2. KAH154-2
  3. KAH132-8
  4. FTRx

> Plate 6.26x103 cells per well in 6-well plates
--> Add 62.5 μL of 1x106/mL suspension to 10 mL plain medium to make 6.25x103/mL dilution
--> Add 1 mL cell dilution to 3 mL plain medium in each well
--> Duplicate samples: 0 dox, 0.5 μg/mL dox*, 1.0 μg/mL dox
Note: *Trying less dox to lessen potential over-expression of neg. ctrls.



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