04/28/10
Minipreps: Used wrong concentration of Amp for colonies. Regrow picked plate and grow two new cultures
- ✓ Cultures: FlpE (2 per colony, 4 total) (use 20 μg/mL Amp/LB)
- ✓ KAH126 and 132 starter cultures: refresh 20% FBS (some aren't growing well)
- ✓ KAH154-159/MV2 stables: split non-clonal back-up stocks in 6-well plates (selective medium)
Note: Pc-ATF + H3me reporter stables: not growing well (back-up stocks also dying); prepare to repeat with less hygro (redo kill-curve?)
|