User:Karmella Haynes/Notebook/BioBrick cloning/2013/09/04

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09/04/13

  • CMV - Pick colonies, make streaks, make liquid cultures



Results from transformation

  • CMV/V0120
    • Transformation was done on 9/03/13
    • Result for plate 1 BBF: many colonies, with some satellite colonies
    • Result for plate 2 BBF (neg. ctrl): 5 colonies, but okay result
    • Result for plate 1 PK: many colonies, with some satellite colonies
    • Result for plate 2 PK (neg. ctrl): many colonies, did not work as expected

Samples for Liquid Cultures

  1. Culture 1 = colony 1 from plate 1 (BBF), CMV, Amp
  2. Culture 2 = colony 2 from plate 1 (BBF), same



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