User:Karmella Haynes/Notebook/BioBrick cloning/2011/08/13

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08/13/11

  • ✓ Cultures/ minipreps: KAH234, 235/V0200 (6 total)



Minipreps
> Check with N/S digests
> Picked 4 KAH235 colonies since there wasn't good separation between the old vector and the insert (8/12/11)

Reagent Volume Expected:
1,2. KAH234/V0200 = 4442, 1041
3-6. KAH235/V0200 = 4442, 2787
Miniprep digests 8/13/11
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.


--> KAH235/V0200 failed. Try again with new KAH235 digest. Run longer to resolve insert and old vector.



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