User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/08

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07/08/11

  • ✓ Cultures/minipreps: KAH204-209 (2 each)
  • ✓ Site directed mutagenesis: p65 mut DpnI digest & transformation (continued from 7/07/11)



Minipreps
> Check with E/P digests

Reagent Volume Expected:
1,2. KAH204 = 2328
3,4. KAH205 = 1251
5,6. KAH206 = 1551
7,8. KAH207 = 1836
9,10. KAH208 = 1884
11,12. KAH209 = 1932
Miniprep digests 7/08/11
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.



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