User:Karmella Haynes/Notebook/BioBrick cloning/2011/05/28

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05/28/11

  • ✓ Retransformations: Plasmids for miR Sensor transfections
  • ✓ Streak plates: Plasmids for miR Sensor transfections



Retransformations
> Measured concentrations of old minipreps, some don't look good.
> Retransform 2 μL plasmid (+ 8μL dH2O) into DH5α-Turbo (25 μL)
> Spot 10 μL on 1/4 plate (Amp). Streak for single colonies

  1. FC1, 12/2/10
  2. FC1, mini, 2/25
  3. FC2, 12/2/10
  4. FC2, mini, 2/25
  5. FC3, 12/2/10
  6. FC3, mini, 2/25
  7. FC4, 3/13/10
  8. FC31, 3/10/10
  9. FC32, 3/10/10
  10. FC33, 3/10/10
  11. FC34, 3/10/10
  12. KAH118, 1/14/10
  13. KAH118, 2/16/10 (Francesca's)
  14. KAH119
  15. KAH119, 1/15/10 (Francesca's)
  16. KAH100, 12/05/09
  17. KAH101, 12/05/09
  18. KAH121, 12/23/09
  19. KAH121, 1/15/10 (Francesca's)
  20. KAH122, 12/23/09
  21. KAH122, 1/15/10 (Francesca's)
  22. KAH124, 12/23/09
  23. KAH125, 12/23/09
  24. KAH125, 1/15/10 (Francesca's)

--> Re-streak from whatever plates can be found in the cold room


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