User:Karmella Haynes/Notebook/BioBrick cloning/2011/01/18

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01/18/11 ✓ Cultures (6 hours) & minipreps: KAH175-178 (2 each); 3 mL ea. Minipreps > Check with E/P/ScaI digests Reagent Volume Expected: 1,2. KAH175 = 2221 3,4. KAH176 = 3191 5,6. KAH177 = 3384 7,8. KAH178 = 3395 V0120 vector = 1785, 1440 15 μL/lane; 1% agarose DNA(plasmid) 2.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 ScaI 1.0 dH2O 8.5   15 μL --> 37°C/ ~15 min.