User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/16

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04/16/10 ✓ Minipreps: KAH142(10), 146/V0201(10); V0201, MV2 ✓ Cultures: 2 mL 6 hour Turbo, KAH157/MV2(4) ✓ Minipreps: KAH157/MV2(4) Minipreps > Check 1-20 with XbaI digests > Check V0201 w/ XbaI > Check MV2 w/ E/P Reagent Volume Expected: V0201 band = 4516 Insert band = BB size + 641 bp 1-10. KAH142/V0201 = 5212 11-20. KAH146/V0201 = 7027 21. V0201 = 5157 22. MV2 = 4529 15 μL/lane; 1% agarose DNA(plasmid) 2.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 9.5   15 μL --> 37°C/ ~15 min. --> Monday: Check KAH142/V0201 via sequencing Minipreps > Check 1-20 with XbaI digests > Check V0201 w/ XbaI > Check MV2 w/ E/P Reagent Volume Expected: MV2 = 4529 1-4. KAH157/MV2 = 1724 15 μL/lane; 1% agarose DNA(plasmid) 2.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 9.5   15 μL --> 37°C/ ~15 min.