User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/16

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04/16/10

  • ✓ Minipreps: KAH142(10), 146/V0201(10); V0201, MV2
  • ✓ Cultures: 2 mL 6 hour Turbo, KAH157/MV2(4)
  • ✓ Minipreps: KAH157/MV2(4)



Minipreps
> Check 1-20 with XbaI digests
> Check V0201 w/ XbaI
> Check MV2 w/ E/P

Reagent Volume Expected:
V0201 band = 4516
Insert band = BB size + 641 bp
1-10. KAH142/V0201 = 5212
11-20. KAH146/V0201 = 7027
21. V0201 = 5157
22. MV2 = 4529
XbaI digest, etc. 4/16/10
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL

--> 37°C/ ~15 min.

--> Monday: Check KAH142/V0201 via sequencing


Minipreps
> Check 1-20 with XbaI digests
> Check V0201 w/ XbaI
> Check MV2 w/ E/P

Reagent Volume Expected:
MV2 = 4529
1-4. KAH157/MV2 = 1724
E/P digest, etc. 4/16/10
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL

--> 37°C/ ~15 min.



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