User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/22

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03/22/10

  • ✓ Cultures: KAH142/V0201 (4)
  • ✓ Assembly: KAH143



Assemblies

  1. KAH143: DT03/(E/S)/1813 ✓ + KAH010/(E/X)/~3234 ✓

--> DT03 = 27.7 ng/μL
--> KAH010 = 25.1 ng/μL


> Ligations

Ligation Plate results (lig : neg crtl) 03/23/10
1. DT03/(E/S)/1813, 28 ng + KAH010/(E/X)/~3234, 25 ng KAH143 10:1 (Pick 4)
2. KAH010(E/X)/ 25 ng ~10 colonies
  1 2
Insert DNA 1.0 ---
Vector DNA 1.0 1.0
2x lgn buf (Roche) 5.0 5.0
T4 ligase (NEB) 1.0 1.0
dH2O 2.0 3.0
  10 μL 10 μL

--> R.T./ ~10 min., Add 50 μL Z-DH5α


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