User:Karmella Haynes/Notebook/BioBrick cloning/2010/02/23

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02/23/10

  • ✓ Minipreps: DPRE (4)
  • ✓ Sequencing order: DPRE clones

Minipreps
> Check with E/S and E/P digests

Reagent Volume Expected:
1,3,5,7. DPRE (E/S) = 1740
2,4,6,8. DPRE (E/P) = 1001, 662, 77
E/S and E/P digest 2/23/10
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.

--> Send all 4 off for sequencing.



Sequencing
--> Genewiz standard sequencing service

  1. DPRE-01 P0001 (fwd): good (keep)
  2. DPRE-01 P0002 (rev): good
  3. DPRE-02 P0001 (fwd): good
  4. DPRE-02 P0002 (rev): good
  5. DPRE-03 P0001 (fwd): good
  6. DPRE-03 P0002 (rev): good
  7. DPRE-04 P0001 (fwd): good
  8. DPRE-04 P0002 (rev): good


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