User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/27

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01/27/10

  • ✓ Minipreps: KAH140, 141/ V0201 (2 each)

Minipreps
> Check with XbaI digest

Reagent Volume Expected:
Vector = 4497
1,2. KAH140/V0201 = 3599
3,4. KAH141/ V0201 = 3187
XbaI digest 1/27/10
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
XbaI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.

--> Got mixed up somewhere along the way. Should have cloned KAH139 and 140 into V0201. KAH141 already done on 1/23.
--> Assemble KAH139/V0201 tomorrow



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