User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/31

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12/31/09 ✓ Minipreps: KAH117(2), 118(2), 119(4); KAH94, 95/JDS43(6 each) ✓ Cultures: KAH117 (4) Minipreps > Check KAH117-119 w/ E/P > Check KAH94, 95/JDS43 w/ PstI Reagent 1-8 9-20 Expected: 1,2. KAH117 = 7758 3,4. KAH118 = 7930 5-8. KAH119 = 8002 9-14. KAH94 fwd = ~8000, 2000, 1800, 500 KAH94 rev = ~5500, 3000, 2000, 1800 15-20. KAH95 fwd = ~8000, 2000, 1800, 500 KAH95 rev = ~5500, 3000, 2000, 1800 15 μL/lane; 1% agarose DNA(plasmid) 4.0 4.0 10X buffer 1.5 1.5 EcoRI 1.0 --- PstI 1.0 2.0 dH2O 7.5 7.5   15 μL 15 μL --> 37°C/ ~30 min. --> Pick 4 more colonies for KAH117 (keep 2 for re-digestion; correct size but low yield?) --> Try re-cloning KAH94, 95/ JDS43(and /JDS42) later