User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/05

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

12/05/09

  • ✓ Cultures: KAH95/MV2(4)
  • ✓ Minipreps: KAH99(2), 100(2), 101(2), 105(2), 106(2), 107(2), 108/MV2(2)

Minipreps
> Check with E/P digests

Reagent Volume Expected:
MV2 = 4529
1,2. KAH99/MV2 = 2913
3,4. KAH100/MV2 = 3085
5,6. KAH101/MV2 = 3157
7,8. KAH105/MV2 = 2794
9,10. KAH106/MV2 = 2788
11,12. KAH107/MV2 = 2966
13,14. KAH108/MV2 = 2960
E/P digest 12/05/09
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.



Personal tools