User:Karmella Haynes/Notebook/ASU courses/2012/01/25

BME194 Syn Bio Demo

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1/25/12 Minipreps: O2-GFP, O1-GFP, Osym-GFP, LacI-O2 for minipreps (5 mL) Transformations: co-transform reporter/ LacI Minipreps > Measure DNA concentration using the Take3 plate (Sarah's Odyssey system) Sample OD 260 260/280 ng/μL 1. O2-GFP 1.613 2.208 80.665 2. O1-GFP 1.911 2.151 95.538 3. Osym-GFP 1.019 2.154 50.961 4. LacI-O2 1.189 2.214 59.459 Transformation > Use 25 ng of each plasmid Plasmid 1 μL Plasmid 2 μL 1. O2-GFP 0.3 LacI-O2 0.4 2. O1-GFP 0.3 LacI-O2 0.4 3. Osym-GFP 0.5 LacI-O2 0.4 > Add 50 μL NEB10B cells (quick and dirty transformation); plate on 100 μg/mL Amp + 50 μg/mL Kan LB agar