User:Karmella Haynes/Notebook/ASU courses/2012/01/24

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1/24/12

  • Transformations: streaks
  • Cultures: O2-GFP, O1-GFP, Osym-GFP, LacI-O2 for minipreps (5 mL); Banana for generator test (5 mL)



Transformations
> Transformations from 1/23 worked!
> Streak single colonies onto agar, grow all day.



Cultures
> Minipreps, 5 mL ea.

  1. O2-GFP (Amp)
  2. O1-GFP (Amp)
  3. Osym-GFP (Amp)
  4. LacI-O2 (Kan)

> Banana generator test (from streaks):

  1. Banana
  2. Banana + 5μL isoamyl alcohol
  3. RFP1
  4. RFP1 + 5μL isoamyl alcohol


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