User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/20

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Entry title

  • See Gel from yesterday PCRs

Gel

  • Lane 1 Ladder
  • Lanes 2-4 Vladimir´s PCR
  • Lane 5 Insert PCR
  • Lane 6 Insert PCR
  • Lane 7 Backbone PCR
  • Lane 8 Backbone PCR
  • Lane 9 Insert Control
  • Lane 10 Backbone Control
  • Lane 11 Ladder

RepC PCR

Reagent Extraction Primordial Plasmid(Given by PhD.Miguel Cevallos Control DNA from Plate1A7
10x PCR buffer Taq Platinum 5μL 5μL 5μL
8mM dNTPs mix 5μL 5μL 5μL
50mM MgCl2 1.5μL 1.5μL 1.5μL
Upper_repC primer 5μM 2μL 2μL 2μL
Low_repC primer 5μM 2μL 2μL 2μL
Template repC region 1μL 1μL 1μL
Platinum Taq DNA-pol 0.5μL 0.5μL 0.5μL
H2O 33.5μL 33.5μL 33.5μL
Total 50μL 50μL 50μL



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