User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/09

Objective

• Clean and know purify of Daniel´s PCR

WetLab

• Purification of PCR product with Roche´s kit of High Pure PCR product.
  • I joined tube A and B and were filled to 100μL

• See purity of PCR cleaning with a gel.
  • Lane 1.5μL Ladder
  • Lane 2.3μL dye & 5μL DNA of cleaned PCR.
  • Lane 3.3μL dye & 5μL Control (Tube C from Daniel´s extraction).

• Ligation of PCR purification:

I made two ligations by distinct ways.

• • DNA insert 5μL
  • DNA vector 1μL
  • Buffer10X 1μL
  • H₂O 1μL
  • T4LigaseNEB 1μL

Second one

• • DNA insert 2μL
  • DNA vector 3μL
  • Buffer10X 1μL
  • H₂O 3μL
  • T4LigaseNEB 1μL