User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/10/10

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October 10th, 2010 1. Make XbaI-PstI double restriction to ΔRBS + GFP E004 Pure PCR product. XbaI-PstI double restriction methods: DNA -> 5 ul Buffer 2 -> 2 ul (10% of total volume) BSA -> 1 ul XbaI -> 1 ul PstI -> 1 ul HPLC -> 10 ul (to complete total volume of 20ul) Incubate at 37º C overnight