User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/07/08

From OpenWetWare

Jump to: navigation, search
iGEM UNAM-Genomics-Mexico Main project page
Previous entry      Next entry

July 8th, 2010

1. Inactivate enzyme from restrictions made on June 7th. Incubate for 20 min at 80ÂșC.


2. Run gel to verify extracted plasmids, PstI and EcoRI-PstI restrictions made on June 7th.

Lanes: 1) Ladder; 2) Plasmid L1; 3) Plasmid L2; 4) Plasmid L4, 5) Plasmid L5; 6) Plasmid L6; 7) p18; 8) p19; 9) p30; 10) L1 PstI; 11) L2 PstI; 12) L4 PstI; 13) L5 PstI; 14) L6 PstI; 15) Ladder.

Lanes: 1) Ladder; 2) p18 PstI; 3) p19 PstI; 4) p30 PstI; 5) L1 EcoRI-PstI; 6) L2 EcoRI-PstI; 7) L4 EcoRI-PstI; 8) L5 EcoRI-PstI; 9) L6 EcoRI-PstI; 10) p18 EcoRI-PstI; 11) p19 EcoRI-PstI; 12) p30 EcoRI-PstI; 13) Ladder.


3. Incubate cells on 5 ml of liquid LB with antibiotic (0.5ul:1ml)

  • L1: p30-MinBP + GFP E0240
  • L2: p30-MinBP + GFP 74 min
  • Standard promoter J23101 + GFP E024
  • GFP reporter Eo240 without promoter



Personal tools