User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2008/09/28
Halogenated natural products from uncultured bacteria
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Recombination experiments started with pOJ436 APRamycin cassette amplification off of PiJ778. Yield was low due to bad enzyme but proceeded with recombination.
Competent cell prep from 10ml SOB with 100mM induction with L-arabinose a-la DK (30C 3hr ~0.5-OD, 30min spike with 100mM arabinose prior to comp cell prep)
Recombination after electroporation for 1hr at 37C. entire reaction plated on amp apra plates (50/50) and grown at 37 ON
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This page was last modified on 29 September 2008, at 16:00.
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