User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/16
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ObjectiveWe are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol DataStock Solution
We run UV-VIS sample of Citrate and HRP with AUNP, and after analyzed the data correcting the absorbance we observed the following graph. For citrate the peak was 523 nm For HRP the peak was 526nm "Figure 1. Absorbance of Citrate-AUNP and HRP-AUNP" The we run the reaction on kinetics, but no activation of the protein was observed as it can be seen in the following figure. "Figure 2. HRP-AUNP luminol |500px " We don't know why no activation of the protein happened in the nanoparticles of gold. |