User:Jason Vicente Mejia/Notebook/H3
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==H3 Staining for Xenopus embryos==
Material: Antibody Rα3. Serine 10, Upstate # 06-570. Antibody is located on Box 1 ( primary antibody).Rabbit needs 2 nd, we have or they have.
Protocol Use antibody Rαh3 at 1/1000. Get the vial from white fridge from adjacent lab and then thaw and get the amount that you need it and then put back. I am going to use this protocol instead of the 12/101. PI after checking the 12/101 staining said that it is ok to use H3 protocol new one with the H3 from Upstate.