User:Jarle Pahr/Growth media

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Compositions of various growth media:


See also http://en.wikipedia.org/wiki/Growth_medium

DM (Davis Minimal broth): Carlton, B. C., and B. J. Brown. 1981. Gene mutation. Pp. 222-242 in P. Gerhardt (ed.), Manual of methods for general bacteriology. American Society for Microbiology, Washington, D.C

See also http://myxo.css.msu.edu/ecoli/dm25liquid.html


http://openwetware.org/wiki/Neidhardt_EZ_Rich_Defined


R2 agar: http://en.wikipedia.org/wiki/R2a_agar

Luria Broth/Lysogeny Broth (LB):

http://en.wikipedia.org/wiki/Lysogeny_broth

TA agar: http://myxo.css.msu.edu/ecoli/taagar.html


In LB medium, exponential phase of E. coli has been estimated to end between OD600 0.6 - 1.0 (Sezonov et al.) According to Sezonov et al., steady state (exponential growth) may end already at OD600 0.3


LA:

To make LA plates, add 20 g agar/L

Psi-B medium:


M9 minimal medium:



EMB Agar: http://www.microbelibrary.org/component/resource/laboratory-test/2869-eosin-methylene-blue-agar-plates-protocol

Burks medium: https://media.vwr.com/stibo/hi_res/8287091.pdf

Media for spiroplasma and/or mycoplasma:

M1D: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=400

Supplies

Agar: http://www.kptkomet.no/produkter/no_89/biologi/vnr/800500-2/


http://www.formedium.com/europe/index.htm


Peptone LP0037: http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=LP0037


Yeast extract LP0021: http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=LP0021&c=UK&lang=EN

Bibliograpy

J Bacteriol. 1974 September; 119(3): 736–747. Culture Medium for Enterobacteria: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC245675/


http://schaechter.asmblog.org/schaechter/2009/11/the-limitations-of-lb-medium.html

Sezonov et al. Escherichia coli Physiology in Luria-Bertani Broth: http://jb.asm.org/content/189/23/8746.full

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