User:Jamie Nunziata/Notebook/Biomaterial Design 2016/2016/03/29

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Objective

  1. Make the functionalized gold nanoparticles in BASIC conditions in hopes of successful synthesis


Protocol

11-Mercaptoundecanamide Gold Nanoparticle Synthesis
A 50μM solution of 11-mercaptoundecanamide was made to be added to pre-made citrate gold nanoparticles. To make the stock, 2.72mg 11-mercaptoundecanamide was dissolved in 2.5mL of hot spring water. 22.5 mL of room temperature citrate gold nanoparticles was brought to pH 10 using 1.0M NaOH and were combined with the 11-mercaptoundecanamide stock solution. This solution is stirred for 30 seconds and left in 4 °C to react overnight.



11-Mertcapto-1-undecanol Gold Nanoparticle Synthesis
A 50μM solution of 11-mercapto-1-undecanol was made to be added to pre-made citrate gold nanoparticles. To make the stock, 2.56mg 11-mercaptoundecanol was dissolved in 2.5mL of hot spring water. 22.5 mL of room temperature citrate gold nanoparticles was brought to pH 10 using 1.0M NaOH and was combined with the 11-mercapto-1-undecanol. This solution is stirred for 30 seconds and left in 4 °C to react overnight.



1-undecanol Gold Nanoparticle Synthesis
A 50μM solution of 1-undecanol was made to be added to pre-made citrate gold nanoparticles. To make the stock, 0.25mL 11-mercaptoundecanamide was combined with 22.5 mL of room temperature citrate gold nanoparticles was brought to pH 10 using 1.0M NaOH. This solution is stirred for 30 seconds and left in 4 °C to react overnight.



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