User:Jamie Nunziata/Notebook/Biomaterial Design 2016/2016/03/15

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Objective

The objective of today's lab was to make the functionalized gold nanoparticles


Protocol

11-Mercaptoundecanamide Gold Nanoparticle Synthesis
A 50μM solution of 11-mercaptoundecanamide was made to be added to pre-made citrate gold nanoparticles. To make the stock, 2.72mg 11-mercaptoundecanamide was dissolved in 2.5mL of hot spring water. 22.5 mL of room temperature citrate gold nanoparticles was combined with the 11-mercaptoundecanamide stock. This solution is stirred for 30 seconds and left in 4 °C to react overnight.



11-Mertcapto-1-undecanol Gold Nanoparticle Synthesis
A 50μM solution of 11-Mertcapto-1-undecanol was made to be added to pre-made citrate gold nanoparticles. To make the stock, 2.72mg 11-Mertcapto-1-undecanol was dissolved in 2.5mL of hot spring water. 22.5 mL of room temperature citrate gold nanoparticles was combined with the 11-Mertcapto-1-undecanol stock. This solution is stirred for 30 seconds and left in 4 °C to react overnight.



1-undecanol Gold Nanoparticle Synthesis
A 50μM solution of 1-undecanol was made to be added to pre-made citrate gold nanoparticles. To make the stock, 0.25mL 1-undecanol was combined with 22.5 mL of room temperature citrate gold nanoparticles. This solution is stirred for 30 seconds and left in 4 °C to react overnight.


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