User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2014/09/30

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Objective UVVis and Fluorescence of re-suspended JCS 1 and JCS 2 Creation of a hot-injection version of this HgS QD synthesis Protocol New BSA Solution (15 mg/mL) .1515 g of BSA in 10.1 mL of HPLC grade water Added 5 mL of 5mM HgNO3 to 5 mL of 15 mg/mL BSA Added 60 μL of 1 M NaOH JCS 4 is being synthesized at room temperature with vigorous stirring JCS 5 is being synthesized at ~50 degrees Celsius (mantle setting 14.5) with vigorous stirring JCS 1 and JCS 2 Data Data updated with JCS 3 Notes The supernatant from the centrifugation of JCS 1 darkened to brown after sitting in the dark in the drawer After speaking with Dr. Hartings, we are going to try to rotovap the water off of the NPs, rather than the precipitation-centrifugation purification procedure.