User:Jacob Esenther/Notebook/581/2014/08/29

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Objective

Complete the synthesis of the polymer and polymer-clay films. Stock solutions of Malachite green were also produced.

Description

  1. Completing film synthesis
    1. Warm 150mL of Sodium Sulfate Solution (see below for details) at 80C for 15 minutes
    2. Place film in Sodium Sulfate Solution (see below for details) at 80C for 1 hour with gentle stirring
    3. Warm 150mL of 1% HCl at 80C for 15 minutes
    4. Place film in 1% HCl Solution (see below for details) at 80C for 30 minutes with gentle stirring
    5. Place film in Sodium Bicarbonate Solution for 15 minutes at room temperature with gentle stirring
    6. Store film in distilled water until further experimentation
  2. Starting new film synthesis
    1. Use the initial film synthesis procedure to make new 10% clay-polyvinyl alcohol film
      1. It was 10% by weight clay because we used 1g of PVA and 0.11g of clay
  3. Malachite Green Solutions
    1. We need to make the following solutions
      1. 25mL 0.20ppm Malachite Green
      2. 25mL 0.50ppm Malachite Green
      3. 25mL 1.1ppm Malachite Green
      4. 25mL 1.4ppm Malachite Green
      5. 25mL 1.7ppm Malachite Green
      6. 50mL 2.0ppm Malachite Green
      7. 50mL 8.0ppm Malachite Green
      8. 50mL 80.0ppm Malachite Green
      9. 50mL 200.0ppm Malachite Green
  4. Preparing the MG Solutions
    1. 200 ppm: 20.00 mg of MG in 100 mL volumetric flask, filled with distilled water
    2. 80 ppm: 20 mL of 200 ppm solution in 50 mL volumetric flask, filled with distilled water
    3. 8 ppm: 2 mL of 200 ppm MG solution in 50 mL volumteric flask, filled with distilled water.
      1. 2 ppm and 1.7 ppm were prepared by Madeleine and Alicia
      2. 1.4 ppm and 1.2 ppm solutions were prepared by Eleni, Melvin and Becky
      3. 0.5 ppm and 0.2 ppm solutions were prepared by Andrew, Tami and Michael
  5. Testing Film Absorption with Malachite Green
    1. Solutions of 2, 8, 80, and 200ppm Malachite Green were prepared.
    2. Films were cut into rounds.
    3. Film rounds were placed into vials with the different Malachite Green solutions and were left to soak.

Stock Solutions

Stock Solutions - provided by Dr. Hartings

  1. 0.2M Sodium Sulfate Solution
    1. I dissolved 22.034g of anhydrous Na2SO4 in 1000mL of water
    2. I added 100mL of concentrated H2SO4 to this solution
    3. This solution will be used to enable the reaction between polyvinyl alcohol and glutaraldehyde in order to cross link the polymer film
  2. 1% wt. HCl Solution
    1. I added 10mL of concentrated HCl to 985mL of water
    2. This solution will be used to enable the reaction between polyvinyl alcohol and glutaraldehyde in order to cross link the polymer film
  3. 0.2M Sodium Bicarbonate Solution
    1. I dissolved 17.042g of NaHCO3 in 1000mL of water
    2. This solution will be used to neutralize the acid in your films

Data

Malachite Green Absorption Prep Image:08-29_Film_Data.jpg

New Polymer Film

  • 1.0091g 22,000 polyvinyl alcohol

New Polymer-Clay Film

  • 1.0068g 22,000 polyvinyl alcohol
  • 0.1144g sodium cloisite

Notes

  • When stirring the films in various solutions, it was noted that the polymer film was torn up by the stir bar. The polymer film was on the bottom of solution and the polymer-clay film was on the top It is recommended to decrease stirring speed, use a smaller stir bar, or try putting the polymer-clay film on the bottom.
  • When cutting the films, they were placed in a petri dish. A falcon tube and spatula were used to cut rounds. This method worked but tore the surrounding film. It is recommended that a sharper instrument be used.
  • Vials for Malachite Green testing were weighed without the lid.




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