User:Igor R. Kuznetsov/Notebook/RBL-2H3/2010/03/17
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U937 Delta ST FPR
Cells suspended in RPMI 1640, 1x107cells / mL. Coverslips coated with Poly-L-lysine (P4832 SIGMA) for 30 minutes, washed 2 times with PBS. Plated media with cells for 30 minutes, then washed with PBS two times to remove suspended cells.
Sample 1: PBS, left at room temperature for 30 minutes
Sample 2: N-fMLFF 10 − 7μM in PBS, left at room temperature for 30 minutes