User:Helen L. Slucher/Notebook/CHEM-572/2014/02/18

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Objective Make solution of HRP-GNPs using beta method Make Hemoglobin-GNP solution and HRP-GNP solution using alpha method Monitoring Activity of HRP-AuNP complexes HRP-AuNP complex solution from the alpha method and the polydopamine-coated AuNPs were placed in high velocity tubes. The nano particle solutions were spun at 18,000rpm for 3 hours. The supernatants were discarded and the pellets were resuspended in water. The polydopamine-coated AuNPs were stored at 4 degrees Celsius The resuspended HRP-AuNP complex solutions were analyzed for activity using UV-Vis The nano particles were added to hydrogen peroxide and OPD (Ophenylenediamine) Active HRP catalyzes the reaction of Ophenylenediamine with H2O2 to produce 2,3-Diaminophenazine Stock solution of OPD with a concentration of 1mg/mL Stock solution of H2O2 113uL OPD and H2O2 UV/Vis HRP supernatant UV/Vis Series are at 10 minute intervals, starting at 0 GNP UV/Vis