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We are going to perform an electrophoresis with the DNA with produced yesterday by using the PCR technique. This is going to allow us to determine if the PCR performed well or not. If the some DNA have been produced during the PCR, we'll sequence some DNA molecules to check if they contain the mutation we induced.
The blue solution contains some glycerol (to make the DNA going into the gel) and some blue colouring agent (to check that the electrophoresis is running well; and to stop the electrophoresis at the right moment).
The left part of the gel corresponds at the DNA standard. We can observe some DNA in the next weel (between the 6th and the 7th standard band). To conclude, we produced DNA during the PCR. The next step is to check if the mutation is contained into our DNA. To do so, we'll sequence our DNA.