User:Fermenter User/Notebook/MOLD A10

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Mold A10 (F3)

Day 0 (Oct 17th Fri):

      <Sung-Hye>           10/17/2008 (11:08)      Start Autoclave Cycle Started
      <Sung-Hye and Frank> 10/17/2008 (13:00)      Drain PBS, transfer media
      
      <Sung-Hye>           10/17/2008 (13:18)      Start BioXpert software  Recording Time 0:00 
      <Sung-Hye>           10/17/2008 (13:45)      Completion of Bioreactor setup for inoculation
       
      <Frank>              10/17/2008 (16:15)      Inoculation: ( 2,3*10^6/ ml ,   80    ml) Fermentation Time 0:00  
      <Frank>              10/17/2008 (22:06)      Cell counts: ( 0,23*10^6/ ml) Viability (  95 %)  Fermentation Time 5:51

Day 1 (Oct 18th Sat):

      <Frank>              10/18/2008 (16:44)      Cell counts: ( 0,20*10^6/ ml) Viability (  95 %)  Fermentation Time 24:29


Day 2 (Oct 19th Sun):

      <Frank>              10/19/2008 (14:30)      Cell counts: ( 0,21*10^6/ ml) Viability (  95 %)  Fermentation Time 46:15


Day 3 (Oct 20th Mon):

      <Frank>              10/20/2008 (13:30)      Cell counts: ( 0,28*10^6/ ml) Viability (  95 %)  Fermentation Time 69:15


Day 4 (Oct 21st Tue):

      <Sung-Hye and Frank> 10/21/2008 (13:18)      Finish BioXpert
                           10/21/2008 (13:30)      2 x UPS are installed
                           10/21/2008 (13:22)      Start BioXpert
      <Frank>              10/21/2008 (13:30)      Cell counts: ( 0,50*10^6/ ml) Viability (  95 %)  Fermentation Time 93:15


Day 5 (Oct 22nd Wed):

      <Frank>              10/22/2008 (13:45)      Cell counts: ( 0,57*10^6/ ml) Viability (  95 %)  Fermentation Time 117:30


Day 6 (Oct 23rd Thu):

      <Frank>              10/23/2008 (13:00)      Cell counts: ( 0,99*10^6/ ml) Viability ( 95  %)  Fermentation Time 140:15


Day 7 (Oct 24th Fri):

      <Frank>              10/24/2008 (13:50)      Cell counts: (1,60*10^6 / ml) Viability ( 95  %)  Fermentation Time 165:35


Day 8 (Oct 25th Sat):

                           10/25/2008 (16:47)      O2 valve opened 100% 
      <Frank>              10/25/2008 (17:00)      Cell counts: ( 2,33*10^6/ ml) Viability (  95 %)  Fermentation Time 192:45
                           impeller speed increased to 200 rpm

Day 9 (Oct 26th Sun):

      <Frank>              10/26/2008 (16:00)      Cell counts: ( 1,93*10^6 / ml) Viability (  90 %)  Fermentation Time 215:45
                           increased O2 flow to about 200ml/min

Day 10 (Oct 27th Mon):

      
                           10/27/2008 (5:00 - 8:00)  HVAC system test (No access to building, No expected power shut-down)
      
      <Frank>              10/27/2008 (12:00)        Cell counts: (1,73*10^6 / ml) Viability ( 85  %)  Fermentation Time 235:45
      <Sung-Hye>           10/27/2008 (13:45)        Terminate Fermentation   Fermentation Time 237:30


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