User:Fermenter User/Notebook/MAUK A9

From OpenWetWare

Jump to: navigation, search
Customize your entry pages


Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

MAUK A9


Day -1 (Mar 23 Mon):

   <Fred>                3/23/2009 14:45 Bring media to fermenter room
   <Sung-Hye>            3/23/2009 14:50 Assemble bioreactor 
                         3/23/2009 14:50 Add 1 ml of Antifoam
   <Sung-Hye>            3/23/2009 14:51 Start Sterilization (Liq 45 min)
                         3/23/2009 00:00 Cooling of bioreactor (Temp setup)
                         3/23/2009 00:00 Start Fermentation Software for Fermenter #7 Record 0:00  
                         3/23/2009 00:00 Start DASGIP OD module 
                         3/23/2009 00:00 Purge with < 0.5 L/min Air
                         3/23/2009 00:00 Calibrate dO2 probe                    
                         3/23/2009 00:00 Connect Acid (500 ml) and Base (400 ml), prime pumps
                         3/23/2009 00:00 Start pH control 
                         3/23/2009 00:00 Set dO2

Day 0 (Mar 24 The):

   <Sung-Hye>            3/24/2009 00:00 Autozeroing OD probe 
                         3/24/2009 00:00 Completion of bioreactor set up

------------------------ <Ready for Inoculation> ------------------------------------

<Fred> 3/24/2009 00:00 Inoculation: (?? ml) Fermentation 0:00 <Fred> 3/24/2009 00:00 Sampling Fermentation 00:00 F7: OD (??)
<Fred> 3/24/2009 00:00 Sampling Fermentation 00:00 F7: OD (??)
<Fred> 3/24/2009 00:00 Sampling Fermentation 00:00 F7: OD (??)
<Fred> 3/24/2009 00:00 Sampling Fermentation 00:00 F7: OD (??)
------------------------ <Induction> ------------------------------------------------ <Sung-Hye> 3/24/2009 00:00 Change temperature <Sung-Hye> 3/24/2009 00:00 Change dO2 <Sung-Hye> 3/24/2009 00:00 Add inducer <Fred> 3/24/2009 00:00 Sampling Fermentation 00:00 Induction 00:00 F7: OD (??)
<Fred> 3/24/2009 00:00 Sampling Fermentation 00:00 Induction 00:00 F7: OD (??)
<Fred> 3/24/2009 00:00 Sampling Fermentation 00:00 Induction 00:00 F7: OD (??)


Day 0 (Mar 25 Wed):

   <Sung-Hye>            3/25/2009 00:00  Havest and Finish Fermentation



Recent changes



Personal tools